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簡(jiǎn)介:
納米金是指金的粒徑為1-100nm之間的粒子,分散在水溶液或有機(jī)溶液中。納米金在一些特定的晶面上存在著表面電子態(tài),其費(fèi)米能級(jí)恰好位于體能帶結(jié)構(gòu)沿該晶向的禁帶中,形成只能平行于表面方向的二維電子云。這是納米金的表面效應(yīng),量子效應(yīng)和宏觀量子隧道效應(yīng)的物理基礎(chǔ)。納米金隨其直徑由大而小呈現(xiàn)深黃色至黑色,納米金在520nm左右可見光譜范圍之間有一個(gè)金的橫向伸縮振動(dòng)吸收峰,吸收波長(zhǎng)隨金粒子粒徑表達(dá)而增加,當(dāng)有納米金棒生成時(shí),在近紅外處就會(huì)出現(xiàn)吸收峰。當(dāng)粒徑從小到大變化時(shí),溶液的顏色這一次表現(xiàn)為深黃色,粉紅色,酒紅色,深紅色,藍(lán)色的變化。隨著金納米粒子的聚沉,吸收峰會(huì)變寬。膠體金的性質(zhì)主要取決于納米金粒子的粒徑及其表面修飾。
球形金納米粒子的部分屬性:
粒徑大小 (nm) | 濃度 (mg/ml) | 粒子數(shù)(Particles/mL) | SPR Wavelength (nm) |
2 | 0.1 | 1.5x1014 | ~510 |
3 | 0.1 | 1.2x1014 | 512~515 |
5 | 0.1 | 5.0x1013 | 515~520 |
10 | 0.1 | 5.7x1012 | 515~520 |
15 | 0.1 | 1.4x1012 | 525 |
20 | 0.1 | 7.0x1011 | 527 |
30 | 0.1 | 2.0x1011 | ~530 |
40 | 0.1 | 9.0x1010 | ~535 |
50 | 0.1 | 4.5x1010 | ~540 |
80 | 0.1 | 2.6x1010 | ~553 |
100 | 0.1 | 1.1x1010 | ~572 |
150 | 0.1 | 5.6x109 | -- |
200 | 0.1 | 1.7x109 | -- |
納米金棒的部分屬性:
長(zhǎng)徑比 | 直徑 (nm) | 濃度 (mg/ml) | SPR Wavelength (nm) | 顏色 |
2.0 | 40 | 0.1 | 660 | 藍(lán)色 |
2.5 | 30 | 0.1 | 657 | 藍(lán)色 |
3.0 | 30 | 0.1 | 700 | 棕黃色 |
3.2 | 25 | 0.1 | 720 | 棕紅色 |
3.5 | 23 | 0.1 | 805 | 酒紅色 |
4.0 | 29 | 0.1 | 760 | 酒紅色 |
4.3 | 25 | 0.1 | 830 | 酒紅色 |
5.0 | 20 | 0.1 | 960 | 藍(lán)色 |
PEG化金納米粒子可以避免納米材料在合成過程中所吸附的表面活性劑(CTAB)所造成的生物毒性,進(jìn)一步提高其水溶性、生物相容性和體內(nèi)循環(huán)時(shí)間,從而獲得更加良好的診斷、成像和光熱治療的能力。通過PEG的官能團(tuán)-COOH,-NH2,-FA,-Biotin等活性基團(tuán)連接生物分子,利用生物分子的特異性識(shí)別作用,使得金納米粒子表面偶聯(lián)特異性分子實(shí)現(xiàn)對(duì)腫瘤的主動(dòng)靶向診斷和治療研究。
PEG化納米金棒的部分屬性:
長(zhǎng)徑比 | 濃度(mg/ml) | SPR Wavelength (nm) |
2.0 | 0.1 | 680 |
2.5 | 0.1 | 680 |
3.0 | 0.1 | 720 |
3.2 | 0.1 | 735 |
3.5 | 0.1 | 820 |
4.0 | 0.1 | 780 |
4.3 | 0.1 | 855 |
5.0 | 0.1 | 980 |
經(jīng)PEG修飾后納米金棒表面會(huì)帶負(fù)電荷
(zeta-AuNRs-CTAB)
(zeta-AuNRs-PEG)
1:聚乙二醇修飾的球形納米金產(chǎn)品:
Gold Nanoparticles,Au-S-PEG-X(末端基團(tuán))
PEG分子量:550/750/1000/2000/3400/5000/10000
X末端基團(tuán):NH2/COOH/MAL/NHS/BIOTIN/SH/N3/Folate/OPSS/FITC/RB/DSPE/Alkyne
SGA/Silane/OH/PLGA/PLA/PCL/CH3
粒徑:5/10/15/20/30/40/50/60/70/80/90/100nm
2:聚乙二醇修飾的納米金棒(AuNRs)產(chǎn)品:
Gold Nanorods,Au-S-PEG-X(末端基團(tuán))
PEG分子量:550/750/1000/2000/3400/5000/10000
X末端基團(tuán):NH2/COOH/MAL/NHS/BIOTIN/SH/N3/Folate/OPSS/FITC/RB/DSPE/Alkyne
SGA/Silane/OH/PLGA/PLA/PCL/CH3
聚乙二醇修飾的納米金及納米金棒的價(jià)格表:
Gold Nanoparticles,Au-S-PEG-Methoxy
PEG分子量:550/750/1000/2000/3400/5000/10000 ,粒徑:5nm-100nm
低濃度:0.1mg/ml 2800元/25ml, 4200元/50ml
高濃度:0.5mg/ml 4200元/25ml, 6500元/50ml
Gold Nanoparticles,Au-S-PEG-Amine(NH2)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Carboxyl(COOH)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Maleimide(MAL)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-NHS (NHS)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Biotin (Biotin)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Azide (N3)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Folic acid (FA)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-OPSS (OPSS)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG- Fluorescein (FITC)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 5200元/25ml, 7800元/50ml
高濃度:0.5mg/ml 7800元/25ml, 9000元/50ml
Gold Nanoparticles,Au-S-PEG-RhodamineB (RB)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 5200元/25ml, 7800元/50ml
高濃度:0.5mg/ml 7800元/25ml, 9000元/50ml
Gold Nanoparticles,Au-S-PEG-DSPE (DSPE)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 5200元/25ml, 7800元/50ml
高濃度:0.5mg/ml 7800元/25ml, 9000元/50ml
Gold Nanoparticles,Au-S-PEG-Cholesterol (CHOL)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 5200元/25ml, 7800元/50ml
高濃度:0.5mg/ml 7800元/25ml, 9000元/50ml
Gold Nanoparticles,Au-S-PEG-Alkyne(ALK)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-OH(OH)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-OH(OH)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-Silane(Silane)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/25ml, 7500元/50ml
Gold Nanoparticles,Au-S-PEG-PLGA(PLGA)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 6500元/25ml, 8300元/50ml
高濃度:0.5mg/ml 8300元/25ml, 9500元/50ml
Gold Nanoparticles,Au-S-PEG-PLA(PLA)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 6500元/25ml, 8300元/50ml
高濃度:0.5mg/ml 8300元/25ml, 9500元/50ml
Gold Nanoparticles,Au-S-PEG-PCL(PCL)
PEG分子量:550/750/1000/2000/3400/5000/10000 粒徑:5nm-100nm
低濃度:0.1mg/ml 6500元/25ml, 8300元/50ml
高濃度:0.5mg/ml 8300元/25ml, 9500元/50ml
Gold Nanorods,Au-S-PEG-Methoxy
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Amine(NH2)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Carboxyl (COOH)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Maleimide (MAL)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-NHS (NHS)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Biotin (Biotin)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Azide (N3)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Folic acid (FA)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 4900元/25ml, 7500元/50ml
高濃度:0.5mg/ml 7250元/10ml, 9700元/25ml
Gold Nanorods,Au-S-PEG-OPSS (OPSS)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG- Fluorescein (FITC)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 4900元/25ml, 7500元/50ml
高濃度:0.5mg/ml 7250元/10ml, 9700元/25m
Gold Nanorods,Au-S-PEG-RhodamineB (RB)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 4900元/25ml, 7500元/50ml
高濃度:0.5mg/ml 7250元/10ml, 9700元/25m
Gold Nanorods,Au-S-PEG-DSPE (DSPE)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 4900元/25ml, 7500元/50ml
高濃度:0.5mg/ml 7250元/10ml, 9700元/25m
Gold Nanorods,Au-S-PEG-Cholesterol (CHOL)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 4900元/25ml, 7500元/50ml
高濃度:0.5mg/ml 7250元/10ml, 9700元/25m
Gold Nanorods,Au-S-PEG-Alkyne(ALK)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-OH(OH)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-Silane(Silane)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 3800元/25ml, 5500元/50ml
高濃度:0.5mg/ml 5300元/10ml, 7500元/25ml
Gold Nanorods,Au-S-PEG-PLGA(PLGA)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 6800元/25ml, 9500元/50ml
高濃度:0.5mg/ml 9200元/10ml, 13000元/25ml
Gold Nanorods,Au-S-PEG-PLA(PLA)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 6800元/25ml, 9500元/50ml
高濃度:0.5mg/ml 9200元/10ml, 13000元/25ml
Gold Nanorods,Au-S-PEG-PCL(PCL)
PEG分子量:550/750/1000/2000/3400/5000/10000 SPR peak600-1000nm
低濃度:0.1mg/ml 6800元/25ml, 9500元/50ml
高濃度:0.5mg/ml 9200元/10ml, 13000元/25ml
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