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Protein crosslinkers蛋白交聯(lián)劑（SPDP/BS3/SMCC/SMPB/GMBS/BSG）

蛋白質(zhì)交聯(lián)劑是一類小分子化合物，具有2個或者更多的針對特殊基團(tuán)（-NH2、-COOH、-HS等）的反應(yīng)性末端，可以和2個或者更多的分子分別偶聯(lián)，從而使這些分子結(jié)合在一起，最終形成共軛蛋白。

Protein crosslinkers are chemical compoundsused to covalently bind, or conjugate, biomolecules together. In proteomics,"protein crosslinking" refers to utilizing protein crosslinkers toconjugate peptides or proteins together or to immobilize proteins to a solidsupport.

Protein crosslinking reagents possessreactive moieties specific to various functional groups (sulfhydryls, amines,carbohydrates, etc.) on proteins, peptides, or other molecular complexes. Theatoms separating a crosslinker’s reactive groups, and eventually the conjugatedproteins, form the “spacer arm”. A zero-length crosslinker refers to proteincrosslinkers that join two molecules without adding additional spacer armatoms. Homobifunctional crosslinkers have the same reactive group on both endsof the spacer arm (i.e., Amine Reactive-Amine Reactive); whileheterobifunctional crosslinkers have different reactive groups on each end of aspacer arm (i.e., Sulfhydryl Reactive-Amine Reactive).

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Zero-LengthCrosslinkers零長度交聯(lián)劑：

產(chǎn)品名稱：Sulfo-NHS

產(chǎn)品簡介：N-hydroxysulfosuccinimide(Sulfo-NHS) is used in conjunction with EDC-HCl (EDAC-HCl) to convert carboxylgroups to Sulfo-NHS esters which react amines. Sulfo-NHS is used as a catalystfor EDC-HCl coupling and greatly increases the efficiency of the reaction.

結(jié)構(gòu)式：

分子量: 217.1

CAS #: 106627-54-7

Alternative Name:Hydroxy-2,5-dioxoyrrolidine-3-sulfonate

HomobifunctionalCrosslinkers 同端雙功能交聯(lián)劑：

產(chǎn)品名稱：BS3

產(chǎn)品介紹：BS3 crosslinker (Bis[sulfosuccinimidyl] suberate; BS3) is a watersoluble homobifunctional pro-tein crosslinker. The 8-atom spacer arm of the BS3crosslinking reagent is non-cleavable and the molecule is not cell membranepermeable. The BS3 protein crosslinker possesses amino reactive Sulfo-NHSesters on both ends of the molecule and can be used to prepare antibody-proteincon-jugates or for crosslinking cell surface proteins.

結(jié)構(gòu)式：

分子量：572 Da

CAS #:82436-77-9

SpacerLength: 11.4 ?

AlternativeNames: Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester); BS3; Sulfo-DSS

產(chǎn)品名稱：BS2G

簡介：BS2G crosslinker(Bis[Sulfosuccinimidyl] glutarate) is a water soluble homobifunctional proteincrosslinker. The BS2G protein crosslinker's 5-atom (7.7 angstrom) spacer arm isnon-cleavable and the molecule is not cell membrane permeable. The BS2Gcrosslinking reagent's Sulfo-NHS esters are amino reactive and can be used tolabel cell surface proteins.

結(jié)構(gòu)式：

Molecular Weight: 530.4

Spacer Length: 7.7 ?

Alternative Names: Glutaric acid bis(3-sulfo-N-hydroxysuccinimide ester); Sulfo-DSG

產(chǎn)品名稱：BSG

簡介：DSG crosslinker (Disuccinimidylglutarate) is a homobifunctional crosslinking reagent that is membranepermeable. DSG crosslinking takes place through amine-reactive NHS esters atboth ends of a 5-atom (7.7 angstrom) spacer arm. DSG must be dissolved in anorganic solvent, such as DMSO or DMF, then added to an aqueous crosslinkingreaction.

結(jié)構(gòu)式：

Molecular Weight: 326.3

CAS #: 79642-50-5

Spacer Length: 7.7 ?

Alternative Name: Di(N-succinimidyl)glutarate

產(chǎn)品名稱：DSP

簡介：Dithiobis(succinimidyl propionate)(DSP) is a homobifunctional crosslinker that is cell membrane permeable. DSPhas amine-reactive N-hydroxysuccimide (NHS) esters at both ends of acleava-ble, 8-atom (12.0 angstrom) spacer arm. DSP contains a reducibledisulfide bond in the spacer arm that make it a good choice for variousapplications.

結(jié)構(gòu)式：

Molecular Weight: 404.4

CAS #: 57757-57-0

Spacer Length: 12.0 ?

Alternative Names: 3,3′-Dithiodipropionicacid di(N-hydroxysuccinimide ester);

產(chǎn)品名稱：DSS

簡介：DSS crosslinker (Disuccinimidylsuberate) is a homobifunctional crosslinker that is cell membrane permeable.DSS has amine-reactive NHS esters at both ends of an 8-atom (11.4 angstrom)spacer arm. The DSS crosslinking reagent must be dissolved in an or-ganicsolvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction.

結(jié)構(gòu)式：

Molecular Weight: 368.4

CAS #: 68528-80-3

Spacer Length: 11.4 ?

Alternative Name: Suberic acidbis(N-hydroxysuccinimide)

產(chǎn)品名稱：DTSSP

簡介：DTSSP crosslinker(3,3′-Dithiobis[sulfosuccinimidylpropionate]) is a homobifunctional proteincrosslinker that is cell membrane impermeable. DTSSP crosslinking reagent hasprimary amine-reactive N-hydroxysulfosuccinimide (Sulfo-NHS) esters at bothends of a cleavable, 8-atom (12.0 angstrom) spacer arm. DTSSP contains areducible disulfide (thiol) bond in the spacer arm that make it a good choicefor various applications.

結(jié)構(gòu)式：

MolecularWeight: 608.5

CAS#: 81069-02-5

Spacer Length: 12.0 ?

產(chǎn)品名稱：EGS

簡介：EGS crosslinker, Ethyleneglycolbis(succinimidylsuccinate), is a water insoluble homobifunction-alcrosslinker. The 12-atom spacer arm of the EGS crosslinking reagent iscleavable. The EGS spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6hours at 37°C. The EGS protein cross-linker possesses amino reactive NHS esterson both ends of the molecule and can be used to label intracellular proteins,since the molecule is cell membrane permeable.

結(jié)構(gòu)式：

MolecularWeight: 456.36

CAS#: 70539-42-3

Spacer Length: 16.1 ?

產(chǎn)品名稱：Sulfo-EGS

簡介：Sulfo-EGS crosslinker, Ethyleneglycolbis(sulfosuccinimidylsuccinate), is a water soluble homo-bifunctionalcrosslinker. The 12-atom spacer arm of Sulfo-EGS crosslinking reagent iscleavable. Sulfo-EGS spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6hours at 37°C. The Sulfo-EGS protein crosslinker possesses amino reactiveSulfo-NHS esters on both ends of the molecule and can be used to label cellsurface proteins, since the molecule is not cell membrane permeable.

結(jié)構(gòu)式：

Molecular Weight: 660.45

SpacerLength: 16.1 ?

Alternative Names: Ethylene glycol bis(sulfosuccinimidylsuccinate);

產(chǎn)品名稱：DSSeb

簡介：DSSeb (Disuccinimidyl sebacateis membranepermeable. DSSeb crosslinks via amine-reactive NHS esters at both ends of a10-atom (14.1 ? ) spacer arm and must dissolved in an organic sol-vent, such asDMSO or DMF, then added to an aqueous crosslinking reaction.

結(jié)構(gòu)式：

MolecularWeight: 396.39

CAS# 23024-29-5

SpacerLength: 14.1 ?

AlternativeNames: Sebacic acid bis(N-hydroxysuccinimide ester);

HeterobifunctionalCrosslinkers異端雙功能交聯(lián)劑：

產(chǎn)品名稱：BMPS

簡介：BMPS is a non-cleavable, water insoluble,heterobifunctional protein crosslinker that is common-ly used to crosslinkhaptens to carrier proteins and enzymes to antibody. The BMPS crosslinker'sspacer arm length of 5.9 angstroms is the shortest of the C3 homolog ofaliphatic spacer series (see also EMCS and GMBS). The BMPS crosslinkingreagent's NHS ester and maleimide reac-tive groups react with amino andsulfhydryl moites, respectively.

結(jié)構(gòu)式：

MolecularWeight: 266.2

CAS#: 55750-62-4

SpacerLength: 5.9 ?

AlternativeNames: N-(beta-Maleimidopropyloxy)succinimide ester

產(chǎn)品名稱：EMCS

簡介：N-(e-Maleimidocaproyloxy)succinimideester (EMCS) is a non-cleavable, water insoluble, hetero-bifunctionalcrosslinker that is commonly used to crosslink haptens to carrier proteins anden-zyme to antibody. The EMCS crosslinker spacer arm is longer than GMBS. TheEMCS NHS es-ter and maleimide reactive groups react with amino and sulfhydrylmoieties, respectively.

結(jié)構(gòu)式：

MolecularWeight: 308.3

CAS#: 55750-63-5

SpacerLength: 9.4 ?

AlternativeNames: N-(epsilon-Malaimidocaproyloxy) succinimide

產(chǎn)品名稱：GMBS

簡介：GMBS is a non-cleavableheterobifunctional protein crosslinker. The GMBS crosslinking reagent iscommonly used to crosslink haptens to carrier proteins and enzymes to antibody.The GMBS has a spacer arm length of 7.3 angstroms and is less immunogenic thanthe crosslinking analog MBS. The GMBS crosslinking reagent's NHS ester andmaleimide reactive groups react with amino and sulfhydryl moites, respectively.

結(jié)構(gòu)式：

MolecularWeight: 280.2

CAS#: 80307-12-6

SpacerLength: 7.3 ?

Alternative Name: 4-Maleimdobutyric acidN-succinimidyl ester

產(chǎn)品名稱：LC-SPDP

簡介：LC-SPDP crosslinker is a cleavable,water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctionalprotein crosslinker. The "long chain" LC-SPDP crosslinking reagenthas a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm ofSPDP.

結(jié)構(gòu)式：

MolecularWeight: 425.5

SpacerLength: 15.7 ?

Alternative Name:Succinimidyl-6-(3-[2-pyridyldithiol-propionamido) hexanoate

產(chǎn)品名稱：MBS

簡介：MBS crosslinker is a non-cleavable,water insoluble, heterobifunctional protein crosslinking rea-gent that iscommonly used to crosslink haptens to carrier proteins and enzymes to antibody.The MBS crosslinking reagent's NHS ester and maleimide reactive groups reactwith amino and sulfhydryl moites, respectively.

結(jié)構(gòu)式：

MolecularWeight: 314.3

CAS#: 58626-38-3

Spacer Length: 7.3 ?

產(chǎn)品名稱：PDPH

簡介：PDPH is a cleavable,heterobifunctional protein crosslinking reagent. The PDPH crosslinker'shydrazide group selectively reacts with oxidized carbohydrates (or carboxylswhen used with EDC); while the pyridinyldisulfide moiety is reactive towardreduced thiols. Thus, PDPH protein crosslinker is an excellent choice for crosslinkingglycoproteins and other polysaccharides.

結(jié)構(gòu)式：

MolecularWeight: 229.3

SpacerLength: 9.2 ?

Alternative Name:3-[2-Pyridyldithio]propionyl hydrazide

產(chǎn)品名稱：SBA

簡介：SBA crosslinker (N-Succinimidylbromoacetate) is a sulfhydryl (thiol) and amino reactive hetero-bifunctionalprotein crosslinking reagent. SBA crosslinker is non-cleavable and is among theshortest amine and sulfhydryl reactive crosslinking reagents known with aspacer arm length of only 1.5 Angstroms.

MolecularWeight: 236.02

CAS#: 42014-51-7

SpacerLength: 1.5 ?

Alternative Names: Bromoacetic acidN-hydroxysuccinimide ester;

產(chǎn)品名稱：SIA

簡介：N-Succinimidyl iodoacetate (SIA) is anon-cleavable, heterobifunctional protein crosslinker. SIA is among theshortest amine and sulfhydryl (thiol) reactive crosslinkers known with a spacerarm length of 1.5 Angstroms.

MolecularWeight: 283.02

CAS#: 39028-27-8

SpacerLength: 1.5 ?

Alternative Names: Iodoacetic acidN-hydroxysuccinimide ester; Succinimidyl iodoacetate

產(chǎn)品名稱：SMCC

簡介：SMCC crosslinker(Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is aheterobi-functional protein crosslinker with a maleimide group that issulfhydryl (thiol; -SH) reactive and a NHS ester group that is amine reactive.The SMCC crosslinking reagent is permeable across the lipid bilayer of the cellmembrane. The 9 atom spacer arm of SMCC is not cleavable.

MolecularWeight: 334.3

CAS#: 64987-85-5

SpacerLength: 8.3 ?

Alternative Names:4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester;

產(chǎn)品名稱：SMPB

簡介：SMPB crosslinker (N-Succinimidyl4-[4-maleimidophenyl]butyrate) is a heterobifunctional pro-tein crosslinkingreagent that is reactive toward sulfhydryl and amino groups. The SMPB pro-teincrosslinker is an extended chain length analog of MBS crosslinker.

MolecularWeight: 356.3

CAS#: 79886-55-8

SpacerLength: 11.6 ?

AlternativeName: 4-(4-Maleimidophenyl)butyric acid N-hydroxysuccinimide ester

產(chǎn)品名稱：SMPH

簡介：SMPHcrosslinker (Succinimidyl-6-[?-maleimidopropionamido]hexanoate) is a water insoluble,heterobifunctional protein crosslinker with a non-cleavable spacer arm lengthof 14.3 angstroms. The SMPH crosslinking reagent has maleimide groups which arereactive to thiols (sufhydryls) and its NHS ester groups react with amines atpH 6.5-7.5 and 7-9, respectively

結(jié)構(gòu)式：

MolecularWeight: 379.4

Alternative Name:Succinimidyl-6-[βmaleimidopropionamido] hexanoate

產(chǎn)品名稱：SPDP

簡介：SPDP crosslinker is a cleavable, waterinsoluble, amino and thiol (sulfhydryl) reactive heterobi-functional proteincrosslinker. The SPDP crosslinking reagent's reactive moites consist of anN-hydroxysuccinimide (NHS) ester that reacts with primary amines. On the otherend of SPDP, the pyridinyldisulfide reacts with sulhydryls to yield areversible disulfide bond.

MolecularWeight: 312.4

CAS#: 68181-17-9

SpacerLength: 6.8 ?

Alternative Name:3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester

產(chǎn)品名稱：Sulfo-LC-SPDP

簡介：Sulfo-LC-SPDP crosslinking reagent isa cleavable, water soluble, amino and thiol (sulfhydryl) reactiveheterobifunctional protein crosslinker.

Sulfo-LC-SPDP crosslinker's reactive moitesconsist of an N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts withprimary amines. The other end of the Sulfo-LC-SPDP crosslinking moleculepyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

MolecularWeight: 527.6

SpacerLength: 15.7 ?

Alternative Name: Sulfosuccinimidyl-6-(3’-[2-pyridyldithio]-propionamido)hexanoate

產(chǎn)品名稱：Sulfo-MBS

簡介：Sulfo-MBS crosslinker(m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester) is a non-cleavable, watersoluble, heterobifunctional protein crosslinking reagent that is commonly usedto crosslink haptens to carri-er proteins and enzymes to antibody. TheSulfo-MBS crosslinking reagent's NHS ester and maleimide reac-tive groups reactwith amine and sulfhydryl moites, respectively.

MolecularWeight: 416.3

SpacerLength: 7.3 ?

Alternative Names: 3-Maleimidobenzoic acidN-hydroxysulfosuccinimide ester;

產(chǎn)品名稱：Sulfo-SANPAH

簡介：Sulfo-SANPAH crosslinker(N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexanoate) is a watersoluble, heterobifunctional protein crosslinker with a spacer arm length of18.2 ?. Sul-fo-SANPAH crosslinking reagent contains an NHS ester that reactswith primary amines and a nitrophenylazide group that is photoreactive at320-350 nm toward amino groups.

MolecularWeight: 492.4

CAS#: 102568-43-4

Spacer Length: 18.2 ?

產(chǎn)品名稱：Sulfo-SMCC

Sulfo-SMCC crosslinker(Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) is a watersoluble, heterobifunctional crosslinker. Sulfo-SMCC crosslinking reagent has an8.3 angstrom non-cleavable spacer arm and its maleimide group reacts withsulfhydryls (thiols), while the Sulfo-NHS ester is reactive toward primaryamines. Sulfo-SMCC can be used to pro-duce antibody-protein crosslinkedconjugates or efficiently label cell surface proteins.

MolecularWeight: 436.4

CAS#: 92921-24-9

Spacer Length: 8.3 ?

DeuteratedCrosslinkers氘代交聯(lián)劑產(chǎn)品：

產(chǎn)品名稱：BS2G-d4

BS2G-d4deuterated crosslinker, Bis(Sulfosuccinimidyl) 2,2,4,4-glutarate-d4, is a watersoluble and cell membrane impermeable crosslinking agent with 4 deuterium atomsto provide a 4 dal-ton shift by mass spectrometry analysis. Use BS2G-d4crosslinking reagent in combination with the non-deuterated form of BS2Gc1126-100mg or c1126-1gm for mass spectrometry studies.

MolecularWeight: 534.37

SpacerLength: 7.7 ?

Alternative Names: Glutaric acid-2,2,4,4-d4bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt

BS3-d4

BS3-d4deuterated crosslinker, Bis(Sulfosuccinimidyl) 2,2,7,7-suberate-d4, is a watersoluble and membrane impermeable crosslinking agent with 4 deuterium atoms toprovide a 4 dalton shift by mass spectrometry. Use in combination withnon-deuterated BS3 c1103-100mg or c1103-1gm for mass spectrometry studies.

MolecularWeight: 576.45

SpacerLength: 11.4 ?

Alternative Names: Suberic acid-2,2,7,7-d4bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt;

DSG-d4

DSG-d4deuterated protein crosslinker (Bis[Succinimidyl] 2,2,4,4-glutarate-d4) is acell mem-brane permeable crosslinking agent with 4 deuterium atoms to provide a4 dalton shift by mass spectrometry analysis. Use the DSG-d4 heavy proteincrosslinker in combination with the light, non-deuterated analog DSG(c1104).for mass spectrometry studies.

MolecularWeight: 330.28

SpacerLength: 7.7?

Alternative Names: Glutaric acid-2,2,4,4-d4bis (N-hydroxysuccinimide ester);

DSS-d4

DSS-d4 deuterated crosslinker(Bis[Succinimidyl] 2,2,7,7-suberate-d4) is a cell membrane perme-ablecrosslinking agent with 4 deuterium atoms that provide a 4 dalton shift by massspectrome-try analysis. Use DSS-d4 heavy crosslinker in combination with DSS,the light non-deuterated analog c1105-100mg or c1105-1gm, for mass specstudies.

Molecular Weight: 372.36

Spacer Length: 11.4 ?

Alternative Names: Suberic acid-2,2,7,7-d4bis (N-hydroxysuccinimide ester);

DSP-d8

DSP-d8deuterated protein crosslinker, Dithiobis(succinimidyl2,2,3,3,6,6,7,7-propionate-d8), is a cell membrane permeable, homobifunctionalprotein crosslinker containing 8 deuterium atoms to provide an 8 dalton shiftby mass spectrometry compared to non-deuterated DSP. DSP-d8 has amine-reactiveNHS esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm.DSP-d8 contains a reducible disulfide bond (SS-bond) in the spacer arm. Use theDSP-d8 deuterated protein crosslinking reagent in combination withnon-deuterated analog DSP for MS studies

Molecular Weight: 412.46

SpacerLength: 12.0 ?

Alternative Names: Dithiobis(succinimidyl)propionate-d8; DSP-d8

BiotinylationReagents生物素交聯(lián)試劑：

產(chǎn)品名稱：BiotinHydrazide

BiotinHydrazide (Biotin-Hz) is a carbohydrate reactive biotinylation reagent. BiotinHydrazide is a great choice for labeling mouse monoclonal antibodies, lectins,sugars, unpaired cytosine residues in nucleic acids, and glycoprotiens.Typically, Biotin Hydrazide is used for coupling gly-coproteins through thecarbohydrate by hydrazone formation.

Molecular Weight: 258.3

CAS#: 66640-86-6

SpacerLength: 15.7 ?

Alternative Name: (+)-Biotin Hydrazide

產(chǎn)品名稱：NHS-Biotin

NHS-Biotin (Succinimidyl Biotin;Succinimidobiotin) is a primary amine reactive biotinylation reagent. TheNHS-Biotin molecule is cell membrane permeable and can be used to covalentlylabel intracellular proteins.

MolecularWeight: 341.4

CAS#: 35013-72-0

SpacerLength: 13.5 ?

Alternative Names: Succinimidobiotin;Biotin-OSU; Biotin N-hydroxysuccinimide ester

產(chǎn)品名稱：NHS-SS-Biotin

NHS-SS-Biotin,Succinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate, is an amine-reactivebiotinylation reagent that contains a reducible disulfide. NHS-SS-Biotin iscell membrane per-meable and has a long chain separating the biotin group fromthe amino-reactive NHS-ester (N-Hydroxysuccinimide ester). This product isoften used to purify an antibody or other protein from a biotin specificaffinity resin like immobilized streptavidin without exposing your protein ofinterest to denaturing conditions.

Molecular Weight: 504.7

CAS#: 142439-92-7

SpacerLength: 24.3 ?

Alternative Name: Biotin disulfideN-Hydroxysuccinimide ester

產(chǎn)品名稱：Sulfo-NHS-Biotin

Sulfo-NHS-Biotin(Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent. It isamine reactive and has a spacer arm length of 13.5?. The Sulfo-NHS-Biotinbiotinylation reagent is ideal for biotinylating antibody or protein that canthen be used for protein detection or immobi-lization. Sulfo-NHS-Biotin isparticularly useful for targeting cell surface proteins.

MolecularWeight: 443.4

CAS#: 119616-92-5

SpacerLength: 13.5 ?

Alternative Names: Biotin3-sulfo-N-hydroxysuccinimide ester sodium salt;

產(chǎn)品名稱：Sulfo-NHS-LC-Biotin

Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl6-(biotinamido) Hexanoate, is a long chain water solu-ble biotinylation reagentthat is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin(b2100-100mg and b2100-1gm), Sulfo-NHS-LC-Biotin is designed to target cell surfaceproteins. The sodium sulfonate groups lend water solubility toSulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cellmembrane.

MolecularWeight: 556.6

CAS#: 128062-22-0

SpacerLength: 22.4 ?

Alternative Name: Biotinamidohexanoic acid3-sulfo-N-hydroxysuccinimide ester sodium salt

產(chǎn)品名稱：Sulfo-NHS-SS-Biotin

Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate(Sulfo-NHS-SS-Biotin) is a cleava-ble, water-soluble, amino-reactivebiotinylation reagent. This molecule is cell membrane imper-meable due to itssodium sulfonate group. Sulfo-NHS-SS-Biotin has a long chain spacer armsep-arating the biotin group from the amino-reactive sulfo-NHS ester moity. Itis possible to cleave the biotin group from the crosslinked target proteinunder reducing conditions, such as in the presence of BME, DTT or TCEP.

MolecularWeight: 606.7

CAS#: 202057-28-1

SpacerLength: 24.3 ?

Alternative Name: Biotin disulfideN-Hydroxysulfosuccinimide ester

OtherModifications其他交聯(lián)劑產(chǎn)品：

產(chǎn)品名稱：HPG

HPG proteinmodification reagent (p-Hydroxyphenylglyoxal) reacts with arginine residues togive a large increase in absorbance at approximately 340 nm. The HPG is abetter choice for ami-no acid side chain modification due to its increasedwater solubility over phenylglyoxal and im-proved oxidation resistance overp-nitrophenylglyoxal. Chemical Formula: C8H8O4

MolecularWeight: 168.2

CAS#: 24645-80-5

Alternative Names: p-Hydroxyphenylglyoxalmonohydrate; 4-hydroxyphenylglyoxal;

產(chǎn)品名稱：SATA

SATA(N-Succinimidyl-S-Acetyl-Thioacetate) is a protein modification reagent used toconvert primary amine groups into protected sulfhydryls (thiol; -SH). Thesulfhydryl is readily made available for use in subsequent reactions afterexposure to hydroxylamine.

CAS#: 76931-93-6

MolecularWeight: 231.2

AlternativeNames: S-Acetylthioglycolic acid N-hydroxysuccinimide ester

產(chǎn)品名稱：Sulfo-NHSAcetate

Sulfo-NHS-Acetateacylating agent (Sulfosuccinimidyl Acetate) is a water soluble.Sulfo-NHS-Acetate irreversibly reacts with primary amines, such as in lysine,at pH greater than 7 to yield the acylated form. Using Sulfo-NHS Acetateprevents polymerization when performing protein crosslinking reactions.

MolecularWeight: 259.17

CAS#: 152305-87-8

AlternativeNames: 1-acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid

產(chǎn)品列表：

Sulfo-NHS   CAS #: 106627-54-7

BS3        CAS#: 82436-77-9

BS2G

BSG              CAS#: 79642-50-5

DSP        CAS#: 57757-57-0

DSS        CAS#: 68528-80-3

DTSSP           CAS#: 81069-02-5

EGS              CAS#: 70539-42-3

Sulfo-EGS    

DSSeb           CAS# 23024-29-5

BMPS            CAS#: 55750-62-4

EMCS            CAS#: 55750-63-5

GMBS           CAS#: 80307-12-6

LC-SPDP             

MBS              CAS#: 58626-38-3

PDPH           

SBA              CAS#: 42014-51-7     

SIA        CAS#: 39028-27-8

SMCC            CAS#: 64987-85-5

SMPB            CAS#: 79886-55-8

SMPH           

SPDP             CAS#: 68181-17-9

Sulfo-LC-SPDP    

Sulfo-MBS    

Sulfo-SANPAH     CAS#: 102568-43-4

Sulfo-SMCC   CAS#: 92921-24-9

BS2G-d4

BS3-d4

DSG-d4

DSS-d4

DSP-d8

Biotin Hydrazide CAS #: 66640-86-6

NHS-Biotin    CAS#: 35013-72-0

NHS-SS-Biotin      CAS#: 142439-92-7

Sulfo-NHS-Biotin CAS #: 119616-92-5

Sulfo-NHS-LC-Biotin    CAS #: 128062-22-0

Sulfo-NHS-SS-Biotin    CAS #: 202057-28-1

HPG              CAS#: 24645-80-5

SATA            CAS#: 76931-93-6

Sulfo-NHS Acetate CAS #: 152305-87-8