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應(yīng)用于藥物載體或生物應(yīng)用的熒光標(biāo)記或基團(tuán)改性的30多種蛋白

時間:2018-05-31 16:26:19       瀏覽:2464

應(yīng)用于藥物載體或生物應(yīng)用的熒光標(biāo)記或基團(tuán)改性的30多種蛋白

西安瑞禧生物科技有限公司是可以提供一些列的蛋白類產(chǎn)品改性 熒光標(biāo)記,偶連抗體 多肽或者聚合物或其他小分子偶連蛋白產(chǎn)品,我們也可以把一些無機(jī)納米顆粒如納米金棒磁性納米顆粒偶連蛋白。

 

我公司可以提供修飾改性熒光標(biāo)記的蛋白有以下:

BSA牛血清白蛋白, HAS人血清白蛋白, Streptavidins鏈霉親和素,Concanavalin A 刀豆球蛋白

Transferrin 轉(zhuǎn)鐵蛋白, Protein A 重組蛋白A, Protein G 重組蛋白G, Actin 肌動蛋白, Histone 組蛋白, Ovalbumin 卵清蛋白, 乳清蛋白lactalbuminHemoglobin 血紅蛋白, Mucin 粘蛋白, Myoglobin 肌紅蛋白, Nucleohistone 核仁蛋白, Casein 絡(luò)蛋白,Lactoferrin乳鐵蛋白,Azo-Casein偶氮酪蛋白,Laminin 層粘蛋白,Keratin azure天青角蛋白,Protamine魚精蛋白,Ferredoxin 鐵氧還蛋白, Green fluorescent protein(GFP)綠色熒光蛋白

 

我們可以提供以下熒光染料標(biāo)記上述蛋白:

FITC/Rhodamine/TRITC/Comarin/Bodipy/ICG/CY3/CY3.5/CY5/CY5.5/CY7/CY7.5/IR-800/IR-825或其他系列IR熒光染料

 

對以上蛋白進(jìn)行活性基團(tuán)改性的基團(tuán)包括有:

活化脂NHS/氨基NH2/生物素Biotin/疊氮N3/醛基CHO/巰基SH/馬來酰亞胺MAL/炔烴Alkyne/DBCO/Vinylsulfone/DOTA/NOTA/TCO/Tetrazine

 

定制偶連的復(fù)合類產(chǎn)品包括有:

以上蛋白可以和納米金或納米金棒偶連或氧化硅納米顆粒偶連

以上蛋白可以和聚合物或者共聚物偶連

以上蛋白可以和點擊化學(xué)類產(chǎn)品或大環(huán)類小分子偶連

以上蛋白可以和其他生物活性產(chǎn)品交聯(lián) 如抗體 多肽 或活性酶

以上蛋白可以根據(jù)文獻(xiàn)制備特殊定制類產(chǎn)品

 

【熒光標(biāo)記人/牛血清白蛋白】:

Bovine serum albumin (BSA) is a slightlyacidic ~66 kd protein. BSA is commonly used in biological research due to itslow cost and inertness. Dilute enzymes can be stabilized by BSA (useful forrestriction digests). BSA is a common blocking agent in western blotting andELISA’s. Although, bovine serum albumin is exceptionally soluble (up to 50% w/vin buffered saline), it is typically used at a concentration of 0.1-1% w/v.

 

產(chǎn)品介紹:

牛血清白蛋白,英文名稱為BovineSerum Albumin,也稱Bovinealbumin,或CohnFraction V,簡稱BSABSA有著極其廣泛的實驗室應(yīng)用,在免疫實驗中常用作封閉劑,包括ELISAWBWestern Blot)。作為載體蛋白,將其交聯(lián)于半抗原和其他弱抗原可以使它們在抗體生產(chǎn)中具有更強(qiáng)的免疫原性。在限制性內(nèi)切酶消化反應(yīng)中,BSA常常被用作一些酶的反應(yīng)穩(wěn)定劑,并且能防止它粘附到管壁和槍頭上。BSA也常用于生物制藥的生產(chǎn)過程或者作為營養(yǎng)物用于細(xì)胞和微生物培養(yǎng)。除此之外,還作為蛋白定量檢測的標(biāo)準(zhǔn)品使用。我們提供的BSA產(chǎn)品,使用優(yōu)質(zhì)牛血漿,利用熱休克法(Heat Shock)法制備而成。

 

牛血清白蛋和人血清白蛋白在藥物靶向和藥物傳遞方面的介紹:

與正常組織相比,腫瘤組織的血管豐富,結(jié)構(gòu)特殊 一個重要的表現(xiàn)就是血管內(nèi)皮間隙較大,這種病變情況下大分子可以透過腫瘤組織血管壁的原因,然而在健康組織的血管中只有小分子可以通過這一內(nèi)皮屏障,通常大分子藥物流體動力學(xué)半徑大于2nm小于10nm 人血清白蛋白的有效半徑是7.2nm 因此可以滲入腫瘤組織而不能進(jìn)入正常組織。

在病例條件下 腫瘤細(xì)胞在生長或轉(zhuǎn)移時,病變細(xì)胞釋放的化學(xué)物質(zhì)和腫瘤衍生物的甲狀腺激素相關(guān)肽均通過產(chǎn)生NO和激活KATP來降低集合淋巴管“節(jié)律泵”的活性,從而影響了淋巴的生成 轉(zhuǎn)運使得腫瘤組織淋巴回流較小,分子量相對較小的物質(zhì)從腫瘤間質(zhì)中清除較快,而大分子被截留,因此大分子物質(zhì)還可以在腫瘤組織中選擇性的聚集。高血管通透性和截留率是白蛋白作為抗腫瘤藥物靶向載體的必要條件,白蛋白的這種效應(yīng)符合大分子物質(zhì)在腫瘤組織的透過性增強(qiáng)及滯留效應(yīng)。

我公司可以提供各種白蛋白熒光探針和活化基團(tuán)改性的牛血清白蛋白和人血清白蛋白產(chǎn)品,并且可以做一些白蛋白復(fù)合定制類產(chǎn)品。

 

已有產(chǎn)品列表:

(BSA-FITC), Bovine serum albumin Fluoresceinlabeled  異硫氰酸熒光素標(biāo)記牛血清白蛋白

(BSA-TRITC), Bovine serum albumin TRITC labeled四甲基落地那么標(biāo)記牛血清白蛋白

(BSA-CY3), Bovine serum albumin CY3 labeled

(BSA-CY5), Bovine serum albumin CY5 labeled

(BSA-CY5.5), Bovine serum albumin CY5.5 labeled

(BSA-CY7), Bovine serum albumin CY7 labeled

(BSA-CY7.5), Bovine serum albumin CY7.5 labeled

(BSA-ICG), Bovine serum albumin ICG labeled吲哚菁綠標(biāo)記牛血清白蛋白-近紅外

(BSA-SH), Bovine serum albumin thiol labeled 巰基功能化牛血清白蛋白

(BSA-Biotin), Bovine serum albumin Biotin labeled生物素修飾牛血清白蛋白

(BSA-MAL), BSA, maleimide function 馬來酰亞胺功能化牛血清白蛋白

(BSA-LA), BSA, linoic acid labeled

(BSA-OA), BSA, oleic acid labeled 油酸包裹牛血清白蛋白

(BSA-N3), BSA, azide labeled  疊氮功能化牛血清白蛋白

IR825-BSA, Bovine serum albumin IR-825 labeled  近紅外染料標(biāo)記牛血清白蛋白

Gold-BSA, Bovine serum albumin Gold labeled  納米金偶連牛血清白蛋白

 

【熒光標(biāo)記和功能化人血清白蛋白】:

Ruixibio provides a variety of chemicallyfunctionalized and bioconjugated Human serum albumin (HSA) with differentfunctionality. These functional HSA products were purified by size exclusionchromatography to remove non-conjugated molecules and ensure adequate applicationsboth in-vitro and in-vivo.

 

(HSA-FITC), Human serum albumin Fluorescein熒光素標(biāo)記人血清白蛋白

(HSA-TRITC), Human serum albumin TRITC  四甲基羅丹明標(biāo)記人血清白蛋白

(HSA-CY5), Human serum albumin CY5

(HSA-CY3), Human serum albumin CY3

(HSA-CY5.5), Human serum albumin CY5.5

(HSA-CY7), Human serum albumin CY7

(HSA-CY7.5), Human serum albumin CY7.5

(HSA-ICG), Human serum albumin ICG  吲哚菁綠標(biāo)記人血清白蛋白-近紅外

(HSA-SH), Human serum albumin thiol   巰基功能化人血清白蛋白

(HSA-Biotin), Human serum albumin Biotin  生物素修飾人血清白蛋白

(HSA-MAL), HSA, maleimide function   馬來酰亞胺功能化人血清白蛋白

(HSA-IR825), Human serum albumin IR-825  近紅外染料標(biāo)記人血清白蛋白

(HSA-Gold), Human serum albumin gold

Human serum albumin (HSA, withoutmodification)

 

【熒光標(biāo)記和功能化卵清蛋白】

蛋清中的主要蛋白質(zhì)包括卵清蛋白、卵轉(zhuǎn)鐵球蛋白、類卵沾蛋白、卵沾蛋白、溶菌酶、球蛋白G2、球蛋白G3等。卵清蛋白等電點4.5,其本質(zhì)是含磷糖蛋白。卵清蛋白是蛋清中的主要蛋白組分,其結(jié)構(gòu)與性質(zhì)會顯著影響蛋清蛋白在食品加工過程中的功能性質(zhì)。因此研究卵清蛋白在熱處理或美拉德反應(yīng)后的變化對了解蛋清蛋白在食品加工過程中的結(jié)構(gòu)與性質(zhì)的變化有重要意義。

 

卵清蛋白是一種優(yōu)質(zhì)蛋白質(zhì),占蛋清蛋白總量的54%-69%,卵清蛋白是典型的球蛋白,分子量為44.5kDa,屬含磷糖蛋白,含有四個自由巰基、385個氨基酸殘基[1]  。這些氨基酸殘基相互纏繞折疊形成具有高度二級結(jié)構(gòu)的球型結(jié)構(gòu),大部分為α-螺旋和β-折疊。卵清蛋白中心有1個二硫鍵和4個巰基,當(dāng)加熱時暴露出來,通過分子間相互作用使卵清蛋白膠體結(jié)構(gòu)變得更穩(wěn)定

 

Ovalbumin (abbreviated OVA) is the mainprotein found in egg white. Ovalbumin has been used in the study of proteinstructure, proteomics and immunology. Ruixibio provides a variety offunctionalized and bioconjugated ovalbumin conjugates.These labeled ovalbuminproducts were purified by size exclusion chromatography to removenon-conjugated molecules and ensure adequate applications both in-vitro andin-vivo.

 

產(chǎn)品列表:

FITC-Ovalbumin, Ovalbumin, FITC conjugate  綠色熒光標(biāo)記卵清蛋白

Rhodamine-Ovalbumin ,Ovalbumin, Rhodaminelabeled 紅色熒光標(biāo)記卵清蛋白

CY3-Ovalbumin, Ovalbumin, CY3 conjugate

Cy5-Ovalbumin ,Ovalbumin, CY5 labeled  藍(lán)色熒光標(biāo)記卵清蛋白

Cy5.5-Ovalbumin ,Ovalbumin, CY5.5 labeled 紫色熒光標(biāo)記卵清蛋白

Cy7-Ovalbumin ,Ovalbumin, CY7 labeled

Cy7.5-Ovalbumin ,Ovalbumin, CY7.5 labeled

ICG-Ovalbumin ,Ovalbumin, ICG labeled  吲哚菁綠標(biāo)記卵清蛋白

Biotin-Ovalbumin ,Ovalbumin, Biotin function  生物素修飾卵清蛋白

Ovalbumin-Thiol, Ovalbumin, Thiol function   巰基化卵清蛋白

Ovalbumin-MAL, Ovalbumin, maleimide function  馬來酰亞胺修飾卵清蛋白

 

【乳清蛋白lactalbumin

該納米載體取材綠色、設(shè)計巧妙,可在腫瘤部位依次針對腫瘤表面過表達(dá)酶、微酸和高谷胱甘肽(GSH)濃度等微環(huán)境實現(xiàn)三重響應(yīng)性釋藥(圖2)。作者首先通過定向酶解奶酪副產(chǎn)物-乳清蛋白獲得兩親性多肽,在水溶液中可自組裝形成30納米單分散膠束peptosome(PS),高效荷載疏水性抗癌藥物姜黃素Cur,解決了姜黃素水溶液難溶的問題,并降低其對正常組織的副作用。隨后他們對其表面修飾功能肽段PePm,其中Pe可靶向乳腺癌表面的ErB2受體,且兼具較好的抗癌轉(zhuǎn)移效果。此外,Pm片段可以根據(jù)腫瘤表面過表達(dá)金屬基質(zhì)蛋白酶MMP的特異性水解,通過交聯(lián)單個載體內(nèi)部自身巰基形成二硫鍵,使PS具有癌細(xì)胞高GSH濃度響應(yīng)釋藥的功能。他們將所構(gòu)建的載體PePm/PS/Cur經(jīng)尾靜脈注射到小鼠體內(nèi)后,經(jīng)EPR效應(yīng)和Pe的靶向效應(yīng)在腫瘤部位高度富集,載體表面Pe肽被MMP水解脫離,使Pe肽留在腫瘤細(xì)胞表面發(fā)揮抑瘤和抗轉(zhuǎn)移效果。PS/Cur繼續(xù)通過“質(zhì)子海綿”效應(yīng)從癌細(xì)胞溶酶體中逃逸,在細(xì)胞質(zhì)內(nèi)通過GSH響應(yīng)逐漸解體,釋放出PS/Cur內(nèi)荷載的姜黃素,發(fā)揮抑制腫瘤的作用。

產(chǎn)品目錄:

FITC-lactalbumin, lactalbumin, FITCconjugate

Rhodamine-lactalbumin , lactalbumin,Rhodamine labeled

CY3-lactalbumin, lactalbumin, CY3 conjugate

Cy5-lactalbumin , lactalbumin, CY5 labeled

Cy5.5-lactalbumin , lactalbumin, CY5.5labeled

Cy7-lactalbumin , lactalbumin, CY7 labeled

Cy7.5-lactalbumin , lactalbumin, CY7.5labeled

ICG-lactalbumin , lactalbumin, ICG labeled

Biotin-lactalbumin, lactalbumin, Biotin function

lactalbumin-Thiol, lactalbumin, Thiol function

lactalbumin-MAL, lactalbumin, maleimide function

 

【熒光標(biāo)記刀豆求蛋白A】:

刀豆球蛋白ACon A),又名刀豆凝集素、刀豆素,是一種植物血凝素,具有強(qiáng)力的促有絲分裂作用,有較好的促淋巴細(xì)胞轉(zhuǎn)化反應(yīng)的作用,其促淋巴細(xì)胞轉(zhuǎn)化最適濃度為40-100μg/ml,能沉淀肝糖原,凝集羊、馬、狗、兔、豬、大鼠、小鼠、豚鼠等動物及人紅細(xì)胞。還能選擇性激活抑制性T細(xì)胞(Ts)細(xì)胞,對調(diào)節(jié)機(jī)體免疫反應(yīng)具有重要作用。因此,通過使用Con A來活化病態(tài)(或老年)時的Ts細(xì)胞這一途徑,可望改觀一些自身免疫性疾病,甚或移植物排斥反應(yīng)或惡性腫瘤的防治前景。

 

刀豆蛋白(ConcanavalinACon A)是從刀豆(Jack bean)中提取出來的凝集素,能沉淀多種糖類,葡聚糖和果聚糖等很多其它多糖,以及免疫球蛋白和血型物質(zhì)等多種糖蛋白,還沉淀肺炎球菌多糖,并能凝集多種紅細(xì)胞。Con A 能和很多細(xì)菌和動物細(xì)胞反應(yīng),能區(qū)分某些正常和腫瘤細(xì)胞,能促進(jìn)細(xì)胞分裂(促有絲分裂作用)。Con A IV 用于淋巴下?lián)苻D(zhuǎn)化實驗,也被用來刺激T細(xì)胞產(chǎn)生IL-1 樣因子,還用于脾細(xì)胞增生,研究激活所必需的配體/受體作用。此外,尚區(qū)分正常分泌ACTH 的垂體細(xì)胞和分泌ACTH 的腺腫瘤細(xì)胞、用于研究糖基化在離子通道調(diào)節(jié)中的作用、用于糖蛋白的分離純化和多糖、糖蛋白糖鏈結(jié)構(gòu)的研究等。

 

1992 年,Tiegs 等成功地應(yīng)用刀豆蛋自A(ConA)誘發(fā)了小鼠特異性肝損傷,這一實驗?zāi)P褪墙陙硇掳l(fā)展起來的由T 淋巴細(xì)胞介導(dǎo)的肝損害,它的建立和應(yīng)用為深入研究肝細(xì)胞損害的細(xì)胞學(xué)與分子學(xué)機(jī)制以及進(jìn)行治療藥物篩選提供了更為方便和理想的實驗動物模型。

 

Concanavalin A (con A) is a homotetrameric,26 kDa lectin derived from the jack bean, Canavalia ensiformis. Concanavalin Abinds to alpha-D glucose and alpha-D-mannose containing saccharides.Concanavalin A requires both Mn2+ and Ca2+ ions as a co-factor for binding.Since each con A has four binding sites, it can agglutinate cells such as redblood cells. Con A can also cause cell surface receptor aggregation which canbe mitogenic for some lymphocytes.

 

產(chǎn)品列表:

Concanavalin A-CY5

Concanavalin A-CY3

Concanavalin A-CY5.5

Concanavalin A-CY7.5

Concanavalin A-CY7

Concanavalin A-Biotin

Concanavalin A-SH

Concanavalin A-TRITC

Concanavalin A-FITC

Concanavalin A-ICG

 

 

 

【熒光標(biāo)記肌動蛋白】

Actin is a major cell cytoskeleton microfilamentprotein that plays an important role in cellular functions such as cellmobility and contraction during cell division. Ruixibio provides a broad rangeof fluorescent or biotin labeled actin bioconjugates. These conjugates includecoumarin labeled actin, fluorescein (FITC, FAM) actins, rhodamine (TRITC, TMR,TAMRA, rhodamine B) actins, cyanine dye (cy3, cy5, cy5.5, cy7) actins and manyother actin conjugates.

 

Actin from rabbit muscle

FITC-Actin, Actin from rabbit muscle,Fluorescein labeled

TMR-Actin, Actin from rabbit muscle,tetramethyl rhodamine (TMR) labeled

TRITC-Actin ,Actin from rabbit muscle,Rhodamine B (TRITC) labeled

CY3-Actin ,Actin from rabbit muscle, Cy3labeled

CY5-Actin ,Actin from rabbit muscle, Cy5labeled

CY5.5-Actin ,Actin from rabbit muscle,Cy5.5 labeled

CY7-Actin ,Actin from rabbit muscle, Cy7labeled

CY7.5-Actin ,Actin from rabbit muscle,Cy7.5 labeled

ICG-Actin ,Actin from rabbit muscle, ICGlabeled

Biotin-Actin, Actin from rabbit muscle, Biotinlabeled

 

 

【熒光標(biāo)記的組蛋白】:

FITC-Histone

Rhodamine-Histone

CY3-Histone

CY5-Histone

CY5.5-Histone

CY7-Histone

CY7.5-Histone

ICG-Histone

 

 

【熒光標(biāo)記血紅蛋白】:

Hemoglobin-CY5

Hemoglobin-CY3

Hemoglobin-CY5.5

Hemoglobin-CY7.5

Hemoglobin-CY7

Hemoglobin-Biotin

Hemoglobin-SH

Hemoglobin-TRITC

Hemoglobin-FITC

 

【熒光標(biāo)記粘蛋白】:

Mucin-CY5

Mucin-CY3

Mucin-CY5.5

Mucin-CY7.5

Mucin-CY7

Mucin-Biotin

Mucin-SH

Mucin-TRITC

Mucin-FITC

 

【熒光標(biāo)記肌紅蛋白】:

Myoglobin-CY5

Myoglobin-CY3

Myoglobin-CY5.5

Myoglobin-CY7.5

Myoglobin-CY7

Myoglobin-Biotin

Myoglobin-SH

Myoglobin-TRITC

Myoglobin-FITC

 

【熒光標(biāo)記核仁蛋白】:

Nucleohistone-CY5

Nucleohistone-CY3

Nucleohistone-CY5.5

Nucleohistone-CY7.5

Nucleohistone-CY7

Nucleohistone-Biotin

Nucleohistone-SH

Nucleohistone-TRITC

Nucleohistone-FITC

 

【熒光標(biāo)記轉(zhuǎn)鐵蛋白】:

 

轉(zhuǎn)鐵蛋白又名運鐵蛋白 transferrinTRFsiderophilin)是血漿中主要的含鐵蛋白質(zhì),負(fù)責(zé)運載由消化管吸收的鐵和由紅細(xì)胞降解釋放的鐵。以TRF-Fe3+的復(fù)合物形式進(jìn)入骨髓中,供成熟紅細(xì)胞的生成

 

藥物的定向運輸和定點給藥系統(tǒng)的研究已取得了可喜的進(jìn)展. 轉(zhuǎn)鐵蛋白-轉(zhuǎn)鐵蛋白受體系統(tǒng)在抗癌藥物和蛋白質(zhì)的定向運輸以及作為基因載體在擴(kuò)散的惡性腫瘤細(xì)胞基因治療中的應(yīng)用研究已接近臨床使用階段. 藥物經(jīng)轉(zhuǎn)鐵蛋白-轉(zhuǎn)鐵蛋白受體介導(dǎo)通過血腦屏障進(jìn)入腦內(nèi)發(fā)揮藥效的給藥途徑的研究也給腦疾病的治療帶來了巨大希望。

 

Fluorescein labeled transferrin, alsocalled FITC transferrin, is one of fluorescent transferrin conjugates providedby Ruixibio. FITC transferrin is a green fluorescent transferrin conjugate withexcitation/emission wavelength at 490 nm/515 nm. Nanocs also provides a numberof orange, red, purple, blue and IR dye labeled transferrin products withexcitation/emission wavelength. These labeled transferrin products werepurified by size chromatography to remove non-conjugated molecules and ensureadequate applications both in-vitro and in-vivo.

 

產(chǎn)品列表:

Transferrin, FITC labeled            

Transferrin, Rhodamine labeled          

Transferrin, Cy3 labeled       

Transferrin, Cy5 labeled       

Transferrin, Cy5.5 labeled           

Transferrin, Cy7 labeled

Transferrin, Cy7.5 labeled                  

Transferrin, Biotin labeled           

Transferrin, ICG labeled             

 

 

【熒光標(biāo)記絡(luò)蛋白】:

Ruixibio offers a variety of fluorescentlylabeled and other functional casein conjugates, which include Fluorescein(FITC) labeled casein, Rhodamine labeled casein, Cy3 labeled casein, Cy5labeled casein, Cy5.5 labeled casein, Alexa fluor and Biotin labeled casein andmany other multifunctional casein products. If you have needs for any specialconjugated casein products not listed here, please call us and let we make foryou!

 

Casein, FITC labeled           

Casein, Rhodamine labeled          

Casein, Cy3 labeled             

Casein, Cy5 labeled             

Casein, Cy5.5 labeled          

Casein, Cy7 labeled      

Casein, Cy7.5 labeled                 

Casein, Biotin labeled          

Casein, ICG labeled      

 

【熒光標(biāo)記親和素和鏈霉親和素】:

 

鏈霉親和素(streptavidin下稱SA)是與親和素(avidin下稱AV)有相似生物學(xué)特性的一種蛋白質(zhì),是streptomyces avidinii菌的分泌物,其分子量及結(jié)合生物素的能力與雞蛋清中的親和素相似,等電點6.0,非特異性結(jié)合遠(yuǎn)比親和素低。

 

鏈霉親和素是四聚體蛋白,大小為66KDa。一分子鏈霉親和素可以高度特異性地與四分子生物素結(jié)合,兩者之間的親和力極為強(qiáng)烈,鏈霉親和素-生物素復(fù)合物的解離常數(shù)處于10-14 mol/L 數(shù)量級,這一性質(zhì)常用于分子生物學(xué)用途。其中一條完整的SA肽鏈中有159個氨基酸殘基,分子量為16450

 

Avidin is a tetrameric protein commonlyisolated from egg whites. Avidin binds to biotin with strength approaching thatof a covalent bond. Disruption of the avidin-biotin bond occurs only underextraordinary conditions, such as pH 1.5 with 6 M guanidium hydrocholoride.Fluorescent avidin can bind to biotinylated antibodies used inimmunohistochemistry and immunocytochemistry. Avidin thiol can bind to goldsurfaces and act as a glue for biotinylated proteins. Avidin agarose is usefulfor efficient removal of biotinylated proteins from a liquid phase.

產(chǎn)品列表:

Neutravidin-thiol 

Streptavidin (without modification) 

Streptavidin-CY5

Streptavidin-CY3

Streptavidin-CY5.5

Streptavidin-CY7

Streptavidin-CY7.5

Streptavidin-ICG

Streptavidin-TRITC

Streptavidin-FITC

Streptavidin-thiol

Streptavidin-azide

Streptavidin, HRP labeled

Streptavidin, maleimide activated

Streptavidin, amine function

Streptavidin, DBCO function

Streptavidin, Dextran Conjugate

Streptavidin, R-phycoerythrin

 

Avidin-CY3

Avidin-CY5

Avidin-CY5.5

Avidin (without modification) 

Avidin-Sepharose

Avidin-thiol

Avidin-FITC

Avidin-TRITC

 

【熒光標(biāo)記和活化改性重組蛋白A】:

Fluorescein labeled Protein A is one of theRuixibio' premier fluorescent Protein A conjugates. Protein A is a 42 kDasurface protein originally found in the cell wall of Stapylococcus aureus.Protein A has strong binding affinity towards immunoglobulins and it has beenused for antibody identification, purification and many other biochemicalresearch uses. Fluorescein protein A is a green fluorescent protein A conjugatethat has excitation/emission wavelength at 495 nm/515 nm. All our protein Aconjugates were purified by size exclusion chromatography to removenon-conjugated molecules and ensure adequate applications both in-vitro andin-vivo.

產(chǎn)品介紹:

天然蛋白AProtein A)是一種發(fā)現(xiàn)于金黃色葡萄球菌的細(xì)胞壁表面蛋白,天然蛋白GProtein G)是一種分離自G型或C型鏈球菌屬的細(xì)胞表面蛋白,二者功能相似,主要通過與免疫球蛋白(Ig)的Fc區(qū)相互作用,可結(jié)合大多數(shù)哺乳動物的IgG。然而兩者結(jié)合特異性上有所不同(詳細(xì)見附錄1,蛋白AG對不同物種Ig的結(jié)合能力總表),如蛋白G對人IgG3,大鼠IgG2a,綿羊IgG1具有很高的親和力,但蛋白A對這些Ig結(jié)合力很弱。蛋白A,蛋白G常共價偶聯(lián)在固體載體如瓊脂糖珠或丙烯酸樹脂小珠上,直接用以做免疫沉淀(IP)或者免疫共沉淀(Co-IP)來進(jìn)行蛋白功能相關(guān)研究,或者直接裝在層析柱上來純化單克隆或者多克隆抗體。

 

西安瑞禧生物科技有限公司使用的是基因改造后的蛋白A和蛋白G,不僅維持其本身的Ig親和特性,同時也去除了天然蛋白本身的非主要結(jié)合域以降低非特異性結(jié)合。本品是同時共價偶聯(lián)了蛋白A和蛋白G的瓊脂糖珠,比單獨的蛋白A或者蛋白G都有更廣的結(jié)合范圍,適合于所有蛋白A瓊脂糖珠和蛋白G瓊脂糖珠單獨可以結(jié)合的Ig,實用性更高。本品為50%的懸液,1 ml Protein A/G 瓊脂糖珠可結(jié)合約25 mgIgG

 

FITC-Protein A  異硫氰酸熒光素標(biāo)記蛋白A

Rhodamine-Protein A  羅丹明標(biāo)記蛋白A

CY3-Protein A  

CY5-Protein A

CY5.5-Protein A

CY7-Protein A

CY7.5-Protein A

ICG-Protein A

Biotin-Protein A  生物素修飾蛋白A

HRP-Protein A   辣根過氧化物酶標(biāo)記蛋白A

Protein A, maleimide function  馬來酰亞胺修飾蛋白A

Protein A, thiol function  巰基修飾蛋白A

Protein A, gold labeled   蛋白A修飾納米金

 

【熒光標(biāo)記和活化改性重組蛋白G】:

Protein G is an immunoglobulin bindingprotein in Streptococci. Protein G binds to the Fc region of antibodies andthereby leaves the Fab region available to still bind antigen. Our recombinantversion has been engineered to lack the albumin binding domains and has a 6xhistidine tag at its N-terminus.

 

FITC-Protein G

Rhodamine-Protein G

CY3-Protein G

CY5-Protein G

CY5.5-Protein G

CY7-Protein G

CY7.5-Protein G

ICG-Protein G

Biotin-Protein G

HRP-Protein G

Protein G, maleimide function

Protein G, thiol function

Protein G, gold labeled

 

Protein AProtein G產(chǎn)品應(yīng)用:

重組蛋白A的C末端含有半胱氨酸,可定向偶聯(lián)到固體分離介質(zhì)上,用于單克隆抗體或多克隆抗體的純化。可結(jié)合大多數(shù)人類和小鼠免疫球蛋白子類(如。,人類IgG1 IgG2IgG4;鼠標(biāo)IgG2 IgG2a,IgG2b IgG3),還結(jié)合牛,幾內(nèi)亞豬,倉鼠,房子,豬,兔子總免疫球蛋白的形式。重組蛋白A可與多種報告分子(熒光染料、酶標(biāo)記、生物素、膠體金、放射性標(biāo)記等)偶聯(lián)并不影響其與抗體結(jié)合的活性。這些偶聯(lián)后的蛋白A衍生物可用于Western-blot、ELISA、免疫組化等的抗體檢測。

 

 

Greenfluorescent protein (GFP)綠色熒光蛋白】:

Green fluorescent protein (GFP) is a 27 kDaprotein derived from Aequorea victoria. It absorbs light in the ultraviolet toblue range and emits green (peak emission 510 nm). GFP is a common reportermolecule for protein expression and visualization of intracellular processes.We produce this protein in E.coli.

 

產(chǎn)品列表:

Green fluorescent protein

GFP-Thiol, Green fluorescent protein, thiol

GFP-Biotin, Green fluorescent protein, Biotin

 

 

IgGfrom human產(chǎn)品:

Human IgG Agarose Beads  Human IgG conjugated to agarose beads

Human IgG Biotin         Human IgG conjugated to biotin

Human IgG CY3           Human IgG conjugated to CY3

Human IgG CY5           Human IgG conjugated to CY5

Human IgG CY5.5        Human IgG conjugated to CY5.5

Human IgG FITC           Human IgG conjugated to FITC

Human IgG               (Minimum 95% purity)

IgGfrom mouse產(chǎn)品:

Mouse IgG CY3            Mouse IgG conjugated to CY3

Mouse IgG CY5            Mouse IgG conjugated to CY5

Mouse IgG CY5.5          Mouse IgG conjugated to CY5.5

Mouse IgG FITC            Mouse IgG conjugated to FITC

Mouse IgG Agarose Beads  Mouse IgG conjugated to agarose beads

Mouse IgG Biotin         Mouse IgG conjugated to biotin

Mouse IgG thiol            Mouse IgG conjugated to thiol

Mouse IgG HRP           Mouse IgG conjugated to HRP

Mouse IgG tetramethylrhodamine       Mouse IgG conjugated to tetramethylrhodamine

Mouse IgG (without modification)

IgGfrom rabbit產(chǎn)品:

Rabbit IgG CY3            Rabbit IgG conjugated to CY3

Rabbit IgG CY5            Rabbit IgG conjugated to CY5

Rabbit IgG CY5.5                Rabbit IgG conjugated to CY5.5

Rabbit IgG FITC            Rabbit IgG conjugated to FITC

Rabbit IgG HRP            Rabbit IgG conjugated to HRP

Rabbit IgG Agarose Beads  Rabbit IgG conjugated to agarose beads

Rabbit IgG Biotin         Rabbit IgG conjugated to biotin

Rabbit IgG thiol         Rabbit IgG conjugated to thiol

Rabbit IgG tetramethylrhodamine       Rabbit IgG conjugated to tetramethylrhodamine

IgG from rabbit (without modification)

 

 

西安瑞禧生物科技有限公司可以提供其他蛋白組學(xué)蛋白交聯(lián)劑部分產(chǎn)品:

BMCC-Biotin

HPDP-Biotin 

Iodoacetyl-LC-Biotin 

Iodoacetyl-PEG2-Biotin

MaleimidePEG11-Biotin

MaleimidePEG2-Biotin

Amine-PEG3-Biotin

Amine-PEG11-Biotin

Pentylamine-Biotin

Sulfo-NHS-LC-Biotin

NHS-Biotin

NHS-LC-Biotin

NHS-LC-LC-Biotin

NHS-PEG12-Biotin

NHS-SS-Biotin
NHS-SS-PEG4-Biotin
PFP-Biotin
Sulfo-NHS-LC-LC-Biotin
TFP-PEG3-Biotin
Alkoxyamine-PEG12-Biotin
Alkoxyamine-PEG4-Biotin
Alkoxyamine-PEG4-SS-PEG4-Biotin
Hydrazide-Biocytin
Hydrazide-Biotin
Biotin-LC-Hydrazide
Hydrazide-PEG4-Biotin
Psoralen-PEG3-Biotin
TFPA-PEG3-Biotin
Photoactivatable Biotin
NHS-Desthiobiotin
Sulfo-NHS-LC-Desthiobiotin
Amine-PEG4-Desthiobiotin
Hydrazide-PEG4-Desthiobiotin
Sulfo-SHPP

DTSSP

BS3  CAS#: 82436-77-9

BS2G

BSG             CAS#: 79642-50-5

DSP        CAS#: 57757-57-0

DSS        CAS#: 68528-80-3

DTSSP    CAS#: 81069-02-5

EGS       CAS#: 70539-42-3

Sulfo-EGS    

DSSeb    CAS# 23024-29-5

BMPS     CAS#: 55750-62-4

EMCS     CAS#: 55750-63-5

GMBS    CAS#: 80307-12-6

LC-SPDP      

MBS       CAS#: 58626-38-3

PDPH           

SBA       CAS#: 42014-51-7

SIA        CAS#: 39028-27-8

SMCC     CAS#: 64987-85-5

SMPB     CAS#: 79886-55-8

SMPH    

SPDP      CAS#: 68181-17-9

Sulfo-LC-SPDP    

Sulfo-MBS

Sulfo-SANPAH            CAS #: 102568-43-4

Sulfo-SMCC                 CAS#: 92921-24-9

Biotin Hydrazide           CAS #: 66640-86-6

NHS-Biotin                  CAS#: 35013-72-0

NHS-SS-Biotin             CAS #: 142439-92-7

Sulfo-NHS-Biotin

HPG                            CAS#: 24645-80-5

SATA                           CAS#: 76931-93-6

Sulfo-NHS Acetate        CAS #: 152305-87-8

Biotin-maleimide         

Biotin ethylenediamine hydrobromide  CAS# 216299-38-6

Biotinamidohexanoic acid hydrazide     CAS#: 109276-34-8

CAS#: 89889-52-1

TSAT

BMPH           CAS#:359436-61-6

EMCH           CAS#:151038-94-7

KMUH           CAS#:1593661-78-9

BMOE           CAS:5132-30-9

BMB              CAS#:28537-70-4

BMH (bismaleimidohexane)         CAS#:4856-87-5

TMEA

DTME           CAS#:71865-37-7

SATP             CAS#:84271-78-3

EMCA           CAS:55750-53-3

Sulfo-NHSN-羥基琥珀酰亞胺磺酸鈉鹽,CAS#: 106627-54-7

Biotin-ONP(+)-生物素-4-硝基苯酯,CAS#: 33755-53-2

Biotin-PFP,五氟苯酚生物素酯/生物素五氟苯酯,CAS#:120550-35-8

6-(馬來酰亞胺基)己酸琥珀酰亞胺酯,CAS#:55750-63-5

6-Maleimidohexanoic acidCAS#55750-53-3

N-Maleoyl-β-alanineMPA),CAS#7423-55-4

4-Maleimidobutyric acidMBA),CAS#57078-98-5

11-馬來酰亞胺十一烷酸 N-琥珀酰亞胺酯,CAS#87981-04-2

2-馬來酰亞胺基乙酸,CAS#25021-08-3

11-Maleimidoundecanoic acidKMUACAS#57079-01-3

Sulfo-EMCS, ε-馬來酰亞胺己酸磺基琥珀酰亞胺酯  CAS#215312-86-0

AMAS, CAS#55750-61-3

1,2-Bis(maleimido)ethane, CAS#5132-30-9

Boc-6-Ahx-OSu, CAS#51513-80-5

N-Boc-2,2-(ethylenedioxy)diethylamine ,CAS#153086-78-3

N,N,N,N-Tetramethyl-p-phenylenediamine,CAS#100-22-1

N-Succinimidyl 6-Biotinamidohexanoate, CAS#72040-63-2

N-(+)-Biotinyl-6-aminohexanoic acid  CAS#72040-64-3

 


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