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RUIXIBIO’ spherical gold nanoparticles are monodispersed gold nanoparticles with narrow size distribution (<15%). These particles were made from chemical reduction in de-ionized water solution. Streptavidin conjugated gold nanoparticles can bind to biotin molecule with high affinity. RUIXIBIO also developed a number of surface coated nanoparticles that provide superior stability and multifunctionaliy. These particles are useful for generating various gold nanoparticle probes to detect various biomolecules in SEM, TEM, light microscopy and blotting